Abstract
[ 125I]VIP (vasoactive intestinal peptide) bound to apical membranes isolated from the bovine tracheal epithelium with a half maximal inhibition by unlabeled VIP (IC 50) of 0.6 × 10 −9M and binding was reversible. Glucagon did not affect [ 125I]VIP binding to the membranes. [ 125I]VIP was covalently cross-linked to tracheal membrane proteins using disuccinimidyl suberate. SDS-polyacrylamide gel electrophoresis of labeled tracheal membranes revealed one major [ 125I]-receptor complex of Mr = 71,000 to which binding of [ 125I]VIP was inhibited by 10 μM unlabeled VIP. These results are consistent with the presence of a specific, high-affinity receptor for VIP, with a Mr = 71,000, in apical membrane vesicles isolated from the bovine tracheal epithelium.
Published Version
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