Abstract
Two cholesterol ester hydrolases (CEH) were detected in the particulate fraction of the rat corpus luteum cell, one sedimenting at low centrifugal forces (between 760 and 25,410 g) and the other at higher centrifugal forces (25,410–124,000 g). The CEH in the fractions sedimenting at low centrifugal forces had an activity of 20–70 pmol/min/mg protein. This enzyme showed a similar sedimentation profile to the lysosomal acid phosphatase enzyme. A second, more active (200–350 pmol/min/mg protein) CEH activity had a different pH optimum and sedimented over higher centrifugal forces. These findings suggest that two CEH can provide cholesterol for steroidogenesis, using as substrate either cholesterol esters stored in the cell, or cholesterol esters derived from plasma lipoprotein.
Published Version
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