Evidence for three carbohydrate prosthetic groups on mouse histocompatibility antigens H-2K d and H-2D b

Abstract

Previous studies have determined that H-2K b molecules have two glycosyl units which are attached at asparagine-86 and asparagine-176. Investigations concerning the number and location of carbohydrate groups in the H-2D b and,H-2K d antigens were carried out on [ 3H]glucosamine-labelled material isolated from EL-4 (H-2 b) and C14 (H-2 d) tumor cells, respectively. The present study, employing CNBr cleavage, indicates that H-2K d and H-2D b have, in addition to those reported for H-2K b, a third glycosyl unit located between residues 229 and the papain cleavage site near residue 281.