Abstract
Intracellular Ca 2+ concentration ([Ca] i) was measured following the activation of an inward Ca 2+ current and subsequent potentiation of an M-type K + current ( I M) in bullfrog sympathetic neurons. Fura-2 was used as an indicator for [Ca] i. The fluorescence ratio at 340 and 380 nm ( F 340/ F 380) was elevated from 0.36 to 1.22 when I M was potentiated by 68% following the Ca 2+ current. Based on the in vivo calibration curve obtained from cells permeabilized with digitonin (20 μM), the F 340/ F 380 value of 1.22 was equivalent to a [Ca] i of 0.97 μM. We therefore propose that a rise in [Ca] i into the micromolar range can lead to the potentiation of I M in amphibian autonomic neurons.
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