Abstract
When mevalonate-[2- 14C] was incubated with seeds of Pinus pinea, 23% of the label in sterols was found in trans-24-ethylidenecholesterol, 12% in a mixture of 24α- and 24β-methylcholesterol, and 65% in 24α-ethylcholesterol. However, when the radioactive substrate was lanosterol-[24- 3H], label appeared only in the 24-ethylidene- (85%) and the epimeric 24-methylsterols (15%). From the ratios of labels in the ethylidene- and methyl-sterols it was possible to show that the tritium in the 24-C 1 -mixture was incorporated only into the 24β-methyl epimer. The labelling patterns are consistent with a pathway to 24β-alkylsterols via Δ 25(27)-sterols bypassing 24-ethylidenesterols and to 24α-alkylsterols via Δ 24(28)-sterols which are isomerized to Δ 24(25)-sterols prior to reduction.
Published Version
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