Abstract
ALTHOUGH many authors have used lysis of eukaryotic cells on alkaline sucrose gradients to measure the size of single-stranded DNA subunits, these studies cannot distinguish a true DNA subunit from a subunit containing non-DNA, alkali-stable linkers. We report here that when Ehrlich ascites tumour (EAT) cell DNA is treated with Pronase before alkali denaturation the size of the released subunit changes from >70S to 26S. T7 DNA present throughout remains unchanged in size, indicating that the change is not due to a soluble nonspecific nuclease.
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