Abstract
SummaryThe one‐way primary equine mixed lymphocyte reaction (MLR) was used to generate allospecific major histocompatibilty complex (MHC) class‐I‐restricted cytotoxicity in vitro. Allospecific cytotoxicity, demonstrated against Con A stimulated peripheral blood mononuclear cells (PBMC) in a 6 h chromium‐release assay, was present from approximately 72 to 168 h of primary culture and peaked between 120 and 168 h. Optimal cytotoxicity was generated at a responder to stimulator ratio of 4:1 and at an effector to target ratio of 50:1. The cytotoxicity was blocked by an anti‐equine leucocyte antigen (ELA) class‐I and an anti‐equine CD8‐homologue monoclonal antibody. An anti‐ELA class‐11 and an anti‐equine CD4‐homologue monoclonal antibody had no significant effect.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call