Abstract
The hypothesis of functional hemizygosity at the emetine-resistant (Emt r, a non-X-linked recessive marker) locus in Chinese hamster ovary (CHO) cells has been examined by segregation analysis. The frequencies and the rates of segregation of the Emt r and Thg r (thioguanine-resistant, an X-linked recessive mutation) markers were determined from hybrids constructed between an Emt r-Thg r CHO cell line and various other Chinese hamster lines (V79, M3-1, CHO, GM7S, CHW and CHL). Thg r segregants were obtained at similar frequencies (10 −2–10 −3) from all the hybrids. The frequency of segregation of the Emt r marker, however, was similar to that of Thg r only in the CHO × CHO hybrids and was much lower (10 −4–10 −6) than the CHO × other Chinese hamster hybrids. Similar results were obtained when the segregation rates for the two markers from various hybrids were determined. These results are consistent with the hypothesis that in CHO cells, the gene responsible for Emt r is present in a single (functional) copy, whereas two copies of this gene are present in other Chinese hamster lines examined.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
