Abstract
Two-dimensional NMR spectroscopy has been used to study the structure and hydrogen bonding scheme of A:G mismatched base pairing in d-GGTAAGCGTACC at pH 5.8. Under the conditions of our study, the molecule forms a B-DNA helix, with the mismatched bases in the A +( anti)-G( syn) conformation. The adenosine exists in the protonated form. The NOESY spectrum in 90% H 2O + 10% 2H 2O has been used to assign all observable imino and amino protons including those involved in the A +( anti)-G( syn) base pair. Both the proton donors in the A:G mismatched inter-base hydrogen bonding are situated on adenosine.
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