Evaluation the exposure of Formalin as a Disinfectant For Poultry House on Hematological, Biochemical Parameters and Histopathological Examination in Broiler

Abstract

This study was done to evaluate exposure to inhalation of formalin as a disinfectant for poultry house on hematological, biochemical parameters and histopathological examination in the broiler. The used (24) chicken divided into two groups, every group included (12) chicken. The first group (control) and second group exposure to formalin by inhalation for 2 weeks. Blood samples were collected at end of each week for analyzed some hematological such as (PCV, Hb, RBCs, total WBC counts and differential WBC count) and biochemical parameters such as (total protein, albumin, globulin and bilirubin) at the end of each week after 1wk scarified of half number of broiler and remove organs such as (lung, heart, and liver) for histopathological examination and end of 2wk scarified the reminder number of broiler. In our study, the hematological changes revealed significant decreases in PCV, Hb, RBCs, and WBC count after 1wk and 2wk compared with the control group. The total protein, albumin, and globulin showed significant decreases while bilirubin showing significant increasing after 1wk and 2wk of treated group compared with the control group. The differential WBC count showed significant increasing into neutrophils, and monocytes after 1wk while there was significant increasing into eosinophils, basophils, and neutrophils after 2wk compared with the control group. There was significantly decreasing into lymphocytes eosinophils and basophils after 1wk of treated groups but after 2wk the monocytes and lymphocytes that showed significant decreasing. The histopathological examination of lung in treated group after 1wk revealed highly changes that represent by congestion of blood vessels, hemorrhages with thickening of blood vessels walls, thickening into alveolar walls, alveoli filled with erythrocytes and fibrin, hemosiderosis, and hyperplasia of alveolar cells into treated group compared with control group, while after 2wk the changes was emphysema (destruction of alveolar walls) only. The heart revealed congestion of blood vessels after 2weeks. The liver revealed more changes that represented by congestion of central veins and necrosis in the hepatocytes, fibrosis, and hyperplasia into the portal area of the liver tissues compared with control group. Changes into liver after 2wk of treated group showed acute inflammation, congestion of blood vessels, hemorrhage and degeneration of hepatocytes with necrosis into some areas. After 1wk the heart show slightly changes includes congestion of blood vessels and slightly infiltration of inflammatory cells and hemorrhage compared with control group, but after 2wk the heart shows more infiltration of inflammatory cells with fibrosis of treated group compared with control group.