Abstract
Realization in minimizing production cost for in vitro culture had brought to a study on application of P. ostreatus spent mushroom compost (SMC). Sterile nodal explants was inoculated on different treatments with 15 replicates each. Treatments were MS medium supplemented with different concentrations of SMC (1 and 2 %) and Benzylaminopurine (BAP) at 0.01, 0.1 and 1.0 mg/L respectively. Observation on plant growth parameters were done and data was recorded on week eight. It can be concluded that high concentration of BAP (1.0 mg/L) with greater SMC concentrations (2%) had showed highest shoots regeneration (4.59±0.76), shoot length (1.6 ±0.36 cm) and number of leaves (10.33±0.58). This study proved that the interaction between SMC and BAP had formedand influenced the regeneration performance of C. nutans.
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