Abstract
DOGR1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOGR1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l-1 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOGR1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOGR1 gene and 2-DOG for regenerating transgenic oil palm.
Highlights
Oil palm (Elaeis guineensis Jacq.), a monocot tree, is the most important economic crop for Malaysia
In this paper we describe the efforts to evaluate the effectiveness of 2-DOG as an alternative selection agent for oil palm transformation
The CaMV35S-DOGR1-Nos fragment was released from pBINARDOG by HindIII and EcoRI digestion, and cloned into pBI35SGUS releasing the DNA fragment of CaMV35S-GUS-Nos at similar sites to generate pBIDOG (Figure 1). pBIDOG was transformed into E. coli and later mobilized into Agrobacterium tumefaciens strain LBA4404 via electroporation and used as a vector for oil palm transformation
Summary
Oil palm (Elaeis guineensis Jacq.), a monocot tree, is the most important economic crop for Malaysia. Palm oil produced from this tree contributes for around 30% of total world’s vegetable oil production. Being a major crop for the country, the oil palm industry has to remain competitive by increasing yield per unit area as well as producing novel high-value products using approaches such as conventional breeding and genetic engineering (Murphy, 2014; Parveez et al, 2015a). The main target of oil palm genetic engineering is increasing the oleic acid content at the expense of palmitic acid (Ravigadevi et al, 2009). Other targets are increasing specialty fatty acids such as ricinoleic, palmitoleic, and stearic, increasing lycopene content as well as synthesizing biodegradable plastics. In order to achieve the above target, construction of transformation vectors for various
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