Evaluation on the effect of Common Food Additive by Cell Based Assay

Abstract

Maltol is a sugar used as common food additive. It is used extensively as a food preservative, antioxidant, and also in cosmetic and pharmaceutical formulations. The aim of the study is to estimate the purity of the drug using quantitative method of analysis, to evaluate the effect of common food additive such as maltol by using MTT assay. By using L6 myoblast cell line, the viability of the cell was determined. Determination of ash values is valuable for the assessment of purity of sample and identified with distinguishing additional earthy matters. Ash values such as total, acid-insoluble and water-soluble ash were worked out as 3.2, 0.965, 3.57 respectively. Extractive values indicate the nature of basic constituents of the sample and it was noticed by alcohol and water. Unnecessary woody material was examined by the method of crude fiber content and was found to be 3.29 and 6.23% of crude fiber. The percentage cell viability was high at the concentration of 62.5μg/ml for the drug maltol by MTT assay.