Abstract
Suosuo grape (Vitis vinifer L) is traditionally used as a therapeutic agent for measles and hepatitis by the ethnic Uighurs. This work aimed to investigate the anti-HBV effect of total triterpene (VTT), total flavonoids (VTF) and total polysaccharides (VTP) from Suosuo grape, and their synergistic effects were also tested. The viral antigens of cellular secretion, HBsAg and HBeAg, were determined by enzyme linked immunosorbent assay (ELISA).The quantity of HBV-DNA released in the supernatant was assayed by real-time PCR. It was found that it effectively suppressed the secretion of HBsAg and HBeAg from HepG2.2.15 cells in a dose-dependent manner, as well as the HBV DNA. The results of orthogonal design experiment showed that the combination of VTT 20 μg/mL, VTF 50 μg/mL and VTP 50 μg/mL had the best optimistic inhibitory effects on HBeAg secretion.
Highlights
Hepatitis B virus (HBV) infections remain a major public health problem for nearly 350 million people
Previous studies showed that total triterpene (VTT), total flavonoids (VTF) and total polysaccharides (VTP) from Suosuo grape had significantly protective effects against immunological liver damage induced by Bacille CalmetteGuerin (BCG) plus lipopolysaccharide (LPS) in vitro and in vivo, and its mechanism might be related to clearance of free radicals, decrease of lipid peroxidation products, protection of hepatocytes membranes, improvement of hepatocyte function and regulation of the secretion of immune cytokines and expressions of apoptosis genes [6,7,8,9]
VTT, VTF and VTP from suosuo grape were able to inhibit the expression of HBsAg, HBeAg, and HBV DNA replication in vitro in a dose-dependent manner
Summary
Hepatitis B virus (HBV) infections remain a major public health problem for nearly 350 million people. Immunization against HBV has been widely used in preventing viral infections, current therapies for these diseases have limited efficacy, there is still an urgent need for the development of effective antiviral agents to eliminate HBV or to inhibit. Previous studies showed that total triterpene (VTT), total flavonoids (VTF) and total polysaccharides (VTP) from Suosuo grape had significantly protective effects against immunological liver damage induced by Bacille CalmetteGuerin (BCG) plus lipopolysaccharide (LPS) in vitro and in vivo, and its mechanism might be related to clearance of free radicals, decrease of lipid peroxidation products, protection of hepatocytes membranes, improvement of hepatocyte function and regulation of the secretion of immune cytokines and expressions of apoptosis genes [6,7,8,9]. The aim of this work was to investigate the synergistic inhibitory effects of VTT, VTP and VTF from Suosuo grape on HBV in vitro
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