Evaluation of Triton X-100 Effect on Coagulation Tests

Abstract

CONTEXT:Triton X-100 is a non-ionic surfactant that has been proposed as a virus inactivator in a laboratory setting for it causes cell lysis through lipid membrane disruption without denaturing proteins. Very few studies have tried to evaluate its effect on laboratory tests, especially on coagulation tests that require a phospholipid subtract.METHOD:Plasma samples were collected. Triton X-100 was spiked at a concentration of 0,25% and specimens were incubated for an hour. We performed international normalized ratio (INR), activated partial thromboplastin time (aPTT), thrombin time (TT), fibrinogen dosing and D-Dimer dosing, comparing the standard plasma with the triton X-100 exposed plasma. We also dosed coagulation factors, and performed dilution and lupus anticoagulant studies. Finally, we compared prothrombin time and aPTT using reagents from various manufacturers.RESULTS:We observed an important difference for the INR and the aPTT with addition of triton x-100. A slight decrease of an average of 6.6 to 16.4% was also found in factor assays. Differences in the INR and the aPTT were dependent respectively on the ISI and the phospholipids content of the reagent.CONCLUSION:The addition of triton x-100 significantly modifies INR and aPTT results with every reagent tested. The impact on TT and fibrinogen is not clinically relevant, and the D- Dimer dosage is not affected. The slight decrease in all coagulation factors does not explain those results. The interference could result from an interaction with phospholipids, calcium or some enzymatic reactions caused by the presence of triton x-100 in the analyzed specimen.TableTestMeanMeanMean of differencep-value (paired t-test)without Tritonwith TritonINR1,592,8768,80%p<0,001aPTT31,59 sec61,80 sec86,10%p<0,001TT16,82 sec17,42 sec3,50%p<0,001Fibrinogen3,27 g/L3,21 g/L-0,70%p=0,001D-Dimer2897 mcg/L2905 mcg/L4,30%p=0,697 DisclosuresNo relevant conflicts of interest to declare.