Evaluation of Tissue Response in Sites Sutured with Cyanoacrylate and Submitted to Low Power Laser Therapy: A Randomized Clinical Trial

Abstract

This study aimed to qualitatively evaluate the inflammatory response in sites sutured with nylon and 2-octyl cyanoacrylate and submitted to low-power laser therapy. Background: 2-octyl cyanoacrylate acts as both a bonding agent for incisional wounds and a substitute for conventional suture methods after topical application, forming an occlusive layer that prevents the entry of any exogenous agent into the wound. Tissue reactions such as granuloma formation are cited in the literature as possible outcomes following 2-octyl cyanoacrylate use. Methods: Thirty wistar rats, were randomized into groups of five, of which fifteen were sutured with 2-octyl cyanoacrylate and another fifteen with nylon thread. In the postoperative period, all the animals were submitted to low power laser therapy in a single centralised region, with light perpendicular to the surgical wound, applied every 48 hours via the ArGaAl laser, with a wavelength of 685nm, a dose of 4J/cm2 and power level of 35mW. All the animals were kept in separate cages, under similar conditions, and provided with water and ad libitum feed. Animals were euthanized at 1 day (D1), 3 days (D2) and 7 days (D3). Tissue samples were removed from the central region of the wound and the slides were stained with hematoxylin and eosin. Results: Histological sections were analysed by evaluating inflammatory parameters and in accordance with a previously standardized scale. Similar inflammatory reactions were observed between sutured wounds with nylon and cyanoacrylate, with no statistically significant differences observed. Conclusions: Results suggest that there is no difference in the association between 2-octyl cyanoacrylate and laser therapy and nylon sutures with and the same As-Ga-Al laser application dosage