Abstract

The contributions of various functional groups to the retention of non-ionogenic compounds such as cardiac glycosides and steroid hormones was studied in reversed-phase liquid chromatography. The dependence of these contributions on the surface chemistry of the adsorbent, the mobile phase composition and column temperature was also investigated. From the dependence of capacity factor k′ of glycosiedes on the column temperature at various mobile phase compositions one can calculate the changes in the enthalpy of adsorption from solution at small coverage. The correlation between the biological activity of the cardiac glycosides and their retention in reversed-phase liquid chromatography was determined. It was demonstrated that this correlation can be used for the evaluation of the biological activity as a function of the contribution of different functional groups to the retention.

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