Abstract
The rule of intersection point between retention of homologues and column temperature in reversed-phase liquid chromatography is reported, based on the thermodynamic theory, with the basic equation of the retention in reversed-phase liquid chromatography, proposed by Vigh and others. We have derived the rule of intersection point between the logarithm of capacity factor and column temperature for homologues in reversed-phase liquid chromatography, and used the experimental data to verify it. The result is satisfactory.
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