Abstract
Measurement of the F 2-isoprostane, 8-epi-PGF 2α is increasingly used as a sensitive and reliable marker of lipid peroxidation in vivo. Because the majority of 8-epi-PGF 2α in plasma is associated with lipoproteins, it is possible that 8-epi-PGF 2α derived from polyunsaturated fatty acid–rich food may become incorporated within these lipoproteins during synthesis and could contribute to the levels detected in plasma. In this study, we evaluated the postprandial effect of a single fast-food meal (McDonald’s Big Mac meal, McDonald’s Corp., London, England) on plasma total 8-epi-PGF 2α in nine healthy subjects. Blood was collected before and 2 h postprandially. 8-Epi-PGF 2α was measured by immunoaffinity extraction and gas chromatography–mass spectrometry. Fasting plasma 8-epi-PGF 2α (875 ± 25 pM) increased postprandially (956 ± 23 pM, p < .05), although no significant change was observed in the normalized concentrations (2.78 ± 0.1 vs. 2.95 ± 0.3 nmol/mmol arachidonic acid). Plasma lipid hydroperoxides, fatty acids, vitamin E, total antioxidant status, cholesterol, and triglycerides were not altered. Plasma glucose increased postmeal (4.4 ± 0.1 vs. 4.9 ± 0.1 mM, p < .05). These results indicate that the overall contribution of this lipid-rich meal to plasma 8-epi-PGF 2α and other lipid peroxidation markers was small.
Published Version
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