Evaluation of the nephroprotective effect of Glycyrrhiza glabra L aqueous extract on CCl4-induced nephrotoxicity in mice

Abstract

In this study, Glycyrrhiza glabra L (GG) aqueous extract was extracted to evaluate its nephroprotective effect on renal structural, hematological, and biochemical changes in CCl4-induced nephrotoxicity in male mice. Fifty male mice were divided into five groups (n = 10); group I served as control, received 1 mL/kg olive oil intraperitoneally and 0.5 mL distilled water through gavage. Group II served as untreated group, received 1 mg/kg CCl4 mixed with olive oil in the ratio of 1:1 intraperitoneally and 0.5 mL distilled water orally. Groups III, IV, V, and VI received CCl4 mixed with olive oil in the ratio of 1:1 intraperitoneally and 30, 90, and 270 mg/kg of GG through gavage for 45 consecutive days. At 45th day, the mice were dissected; blood and kidney samples collected for hematological, biochemical, and histological parameter analysis. The data of this study were analyzed by the SPSS-18 software using one-way analysis of variance (ANOVA) followed by Duncan’s post hoc test (p ≤ 0.05). Different doses of GG (especially GG270) could significantly (p ≤ 0.05) reduce the raised levels of urea, creatinine, WBC, and platelet and increased SOD, CAT, and RBC levels as compared to the untreated group. The weight and volume of the renal structures were decreased significantly (p ≤ 0.05) in several doses of GG as compared to the untreated group. In conclusion, GG has nephroprotective effect, thereby reducing the causation of toxicity in experimental mice.