Abstract
Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription.
Highlights
Acute respiratory infections (ARI) are the leading cause of morbidity and mortality in infants and children worldwide, especially in Southeast Asia and Africa[1,2]
We aimed to evaluate the diagnostic performance of the Luminex xTAG Respiratory Viral Panel (RVP) FAST v2 against a combination of a number of published reference assays on clinical specimens collected from patients with ARI from four provincial hospitals in Vietnam
Comparison of the Luminex xTAG RVP FAST v2 test and references Diagnostic yields of Luminex and reference assays were 66.7% (295/442, 95% CI, 62.1-71.1%) and 54.1% (239/442, 95% CI, 49.3-58.8%), respectively
Summary
Acute respiratory infections (ARI) are the leading cause of morbidity and mortality in infants and children worldwide, especially in Southeast Asia (including Vietnam) and Africa[1,2]. The introduction of multiplex PCR for detection of a panel of respiratory pathogens enables faster results, higher throughput and lower cost[13,14]. Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were
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