Evaluation of the Impact of Biofield Treatment on Physical and Thermal Properties of Casein Enzyme Hydrolysate and Casein Yeas t Peptone

Trivedi Mk,Nayak G

doi:10.4172/2167-065x.1000138

Abstract

In the present study, the influence of biofield treatment on physical and thermal properties of Casein Enzyme Hydrolysate (CEH) and Casein Yeast Peptone (CYP) were investigated. The control and treated samples were characterized by Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC), Thermo Gravimetric Analysis (TGA), particle size and surface area analysis. The FTIR results revealed that biofield treatment has caused reduction of amide group (amide-I and amide-II) stretching vibration peak that is associated with strong intermolecular hydrogen bonding in treated CEH as compared to control. However, no significant changes were observed in FTIR spectrum of treated CYP. The TGA analysis of treated CEH showed a substantial improvement in thermal stability which was confirmed by increase in maximum thermal decomposition temperature (217°C) as compared to control (209°C). Similarly, the treated CYP also showed enhanced thermal stability as compared to control. DSC showed increase in melting temperature of treated CYP as compared to control. However the melting peak was absent in DSC of treated CEH which was probably due to rigid chain of the protein. The surface area of treated CEH was increased by 83% as compared to control. However, a decrease (7.3%) in surface area was observed in treated CYP. The particle size analysis of treated CEH showed a significant increase in average particle size (d50) and d99 value (maximum particle size below which 99% of particles are present) as compared to control sample. Similarly, the treated CYP also showed a substantial increase in d50 and d99 values which was probably due to the agglomeration of the particles which led to formation of bigger microparticles. The result showed that the biofield treated CEH and CYP could be used as a matrix for pharmaceutical applications.

Highlights

Over the last few decades, there has been continuous interest in biodegradable polymers for pharmaceutical and biomaterial applications [1]
The Fourier Transform Infrared (FTIR) spectrum of control Casein Enzyme Hydrolysate (CEH) showed (Figure 1a) an important absorption peaks at 3215 cm-1, 2974 cm-1 which were attributed to -OH and -CH stretching vibration peaks respectively
The -OH stretching vibration peak was shifted to 3199 cm-1 and amide group peaks were shifted to lower wavenumber 1633 cm-1 and 1587 cm-1 respectively. This showed that biofield treatment has induced strong intermolecular hydrogen bonding in treated CEH structure

Summary

Introduction

Over the last few decades, there has been continuous interest in biodegradable polymers for pharmaceutical and biomaterial applications [1]. The food proteins have their inherent ability to interact with wide range of bioactive compounds via functional groups present on their polypeptide structure. This offers the reversible binding of active molecules and protects them until their safe release in the human body [7,8]. Enzyme hydrolysis was recently used to modify the protein structure in order to enhance the functional properties of proteins. It was believed that electromagnetic field exists around the human body and the evidence was found using some medical technologies such as electromyography, electrocardiography, and electroencephalogram This field is known as biofield and the exposure of the said biofield has been referred hereinafter as Biofield treatment. The effects of biofield treatment on two protein based organic compounds (CEH and CYP) are studied and their physicochemical properties are evaluated

Materials and Methods

Results and Discussion

Conclusion