Abstract
Dermatophytosis is considered as one of the most important fungal skin disease affecting both humans a nd animals otherwise its importance as zoonotic diseas e. Treatment of ringworm in cattle is expensive and time consuming especially on herd level. Vaccination can be a giant step forward to prevent the occurrence of mycozoonosis between the affected animals with ringworm and susceptible man. Fifty skins scraping samples were collected from animals (cattle, sheep and calves) suffering from skin lesions suspected t o be ringworm. Then these collected samples were subjected to mycological examination and identification. T. verrucosum is the main causative agent of collected skin scra pings. Two types of vaccines were prepared from this strain (Culture filtrate and formalin ina ctivated vaccines). Rabbit model was used for chall enge testing of the vaccines. The rabbits were separated into four groups as follow: Each group contained 3 rabbits each one was injected subcutaneously with 1 ml of culture filtrate with adjuvant twice for seve n days intervals. Group No. 2 rabbits each one was injecte d intramuscularly with 1 mL formalin-inactivated wi th adjuvant. The 3rd group is control positive and 4th one is control negative. Vaccination programme is done via injection twice with one week interval and the challenge by scarification of the skin with 0.2 mL of 5×10 7 fungal elements/mL. For 3 days. Humeral immune response was assessed by measuring anti-T. verrucosum antibodies using ELISA technique (direct method). By using statistical analysis of ANOVA, the mean optical density of anti- T. verrucosum specific IgG level in rabbits artificially immuniz ed with culture filtrate with adjuvant vaccine significantly increa se from (1.97) post first vaccination to reach (2.4 3) post second vaccination and still high after challenge w ith the virulent T. verrucosum strain (2.32) when compared with control positive (1.57). The results revealed that both type of vaccines induce good hum eral immune response in vaccinated animals. Culture filt rate vaccine used as a treatment in infected farm animals by two doses within 2 weeks and noted that healing occurred.
Highlights
The results revealed that both type of vaccines induce good humeral immune response in vaccinated animals
Culture filtrate vaccine used as a treatment in infected farm animals by two doses within 2 weeks and noted that healing occurred
Twelve rabbits of mixed breeds apparently health and tested for freeing from dermatophytosis weighted from 1 kg to 1.5 kg they feed on special rabbit feed and divided into 4 group, each of which contains 3 rabbits, the first group was given culture filtrate vaccine by 1 mL s/c twice with one week interval 2nd group was given formalin inactivated vaccine by 1 mL s/c twice with one week interval. 3rd group was considered as control positive which challenged only
Summary
Occurrence of mycozoonosis between the affected animals with ringworm and susceptible man in Dermatophytosis is common and clinically regions having predisposing factors such as close contact multifaceted fungal skin disease affecting both humans with the animal, traditional farming and cold winter and animals and causes more economic losses among season (Ozkanlar et al, 2009). There is definitely a need for effective immunotherapy against dermatophytosis was recorded in prophylaxis against the disease as hygienic and Russia. They applied a dermatophyte-killed vaccine preventive measures often fail Gudding and Lund prepared from six dermatophyte species for treatment of (1995). Corresponding Author: Rasha Nabil Zahran, Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Sadat University, Egypt. The present study was planned to cover the following: Isolation and Identification of the main causative agent of dermatophytosis in cattle onto mycological media, Preparation of different types of vaccines from the main isolated common fungi, Measurements of immune response developed against the prepared fungal vaccines and Determination of the immunotherapeutic value of the prepared vaccines in field cases
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