Abstract
The development of adequate methods for monitoring the functional state of neutrophils is an urgent task due to their important role in the development of the inflammatory response. In this study, the functional activity of neutrophils has been assessed using a complex approach, including the registration of generation of ROS by neutrophils as a result of the functioning of the NADPH oxidase complex; degranulation of azurophilic granules; as well as production of AFG, formed in reactions catalyzed by the enzyme azurophilic granules of neutrophils – MPO in the presence of substrates (Н2О2 and halide ions).
Highlights
На основании полученных данных можно заключить, что при необходимости универсальной оценки функциональной активности нейтрофилов наиболее рациональным будет применение флуоресцентного метода с использованием целестинового синего В, позволяющего оценивать продукцию клетками гипогалоидных кислот и их производных, необходимыми условиями образования которых являются высвобождение МПО в результате дегрануляции и наличие ее субстрата – Н2О2, образующегося при респираторном взрыве нейтрофилов
Сергей Николаевич Черенкевич, доктор биологических наук, профессор, кафедра биофизики, НИЛ биофизики и биотехнологии, Белорусский государственный университет, пр
Summary
EVALUATION OF THE FUNCTIONAL CONDITION OF HUMAN BLOOD NEUTROPHILLS BASED ON THE REGISTRATION.
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