Estimation of the Genotoxicityof Hydrogen Peroxide and Antioxidant Actionof Halo Acid by the Method of Dna-Cometwith the Use of the Model of Transplantable Cell Cultures

Galina Dudchenko,Olga Verle,Oleg Ostrovskiy,Valerian Verovskiy

doi:10.15688/jvolsu11.2018.1.7

Abstract

In the framework of the study, the degree of defragmentation of DNA by the DNA-comet method is evaluated when exposed to the cell culture of hydrogen peroxide (H2O2), and an in vitro model is developed to evaluate the antioxidant activity of new pharmacological agents. The results of working with cell lines show that the percentage of damage to the genetic material of cells of intact samples does not greatly vary from the method of removing the cellular monolayer from the culture plastic. Concerning the effect of H2O2 as an inducer of oxidative stress on DNA cell damage, the optimal level of DNA defragmentation has been modeled for subsequent studies of the protective action of antioxidants.

Highlights

In the framework of the study, the degree of defragmentation of DNA by the DNA-comet method is evaluated when exposed to the cell culture of hydrogen peroxide (H2O2), and an in vitro model is developed to evaluate the antioxidant activity of new pharmacological agents
The results of working with cell lines show that the percentage of damage to the genetic material of cells of intact samples does not greatly vary from the method of removing the cellular monolayer from the culture plastic
Concerning the effect of H2O2 as an inducer of oxidative stress on DNA cell damage, the optimal level of DNA defragmentation has been modeled for subsequent studies of the protective action of antioxidants

Summary

КРАТКИЕ СООБЩЕНИЯ

ESTIMATION OF THE GENOTOXICITY OF HYDROGEN PEROXIDE AND ANTIOXIDANT ACTION OF HALO ACID. BY THE METHOD OF DNA-COMET WITH THE USE OF THE MODEL OF TRANSPLANTABLE CELL CULTURES. Ostrovskiy Volgograd State Medical University, Volgograd, Russian Federation. Verovskiy Volgograd State Medical University, Volgograd, Russian Federation. Dudchenko Volgograd State Medical University, Volgograd, Russian Federation

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