Abstract
BackgroundThe current study determined the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma (GA).MethodsReal-time quantitative reverse transcription (qRT)-PCR was used to detect the level of lncRNA expression in 145 GA tissues and three GA cell lines, and the correlation between its level and clinicopathologic characteristics and potential corresponding mRNA of TNF receptor-associated factor 4 gene (TRAF4) was then evaluated.ResultsElevated lncRNA AC010761.9 was detected in all 6 GA tissues by previous lncRNA expression profile microarray assay. LncRNA AC010761.9 was over-expressed in 99 of 145 GA tissues (68.3%) with an elevated fold change of up to 35.14 compared to matched paracancerous tissues (p < 0.05), and was also over-expressed in the 3 GA cell lines (MGC803, BGC823, and SGC7901) compared to the normal gastric mucosal epithelial cell line (GES-1 cells; p < 0.05) by qRT-PCR. The elevated expression of this lncRNA was related to tumor size (p = 0.028), degree of differentiation (p = 0.047), and serum carbohydrate antigen (CA19-9) and carcinoembryonic antigen (CEA) concentrations (p = 0.026 and p = 0.037, respectively). Multivariate analysis further confirmed that the expression of lncRNA AC010761.9 was related to the degree of tumor differentiation (p = 0.015). Additionally, the expression of lncRNA AC010761.9 had a positive correlation with the mRNA expression of the potentially associated gene (TRAF4) in GA tissues (r = 0.385, p < 0.01).ConclusionsLncRNA AC010761.9 may be linked to GA progression and is a potential new biomarker for GA.
Highlights
The current study determined the expression and clinical value of long non-coding RNAs (lncRNAs) AC010761.9 in human gastric adenocarcinoma (GA)
LncRNA expression chips showed that lncRNA AC010761.9 is over-expressed in GA tissues The lncRNA microarray chip assays were performed in six GA tissues and six matched paracancerous tissues
The results showed that the level of lncRNA AC010761.9 expression was higher in six GA tissues than the matched non-GA tissues (Fig. 1)
Summary
The current study determined the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma (GA). Patients with advanced-stage gastric cancer frequently die. This malignant illness can occur at any age; gastric cancer usually affects the elderly. Recent studies have shown that long non-coding RNAs (lncRNAs) are closely related to a variety of human cancer. Emerging evidence has shown that gastric cancer occurrence and progression are accompanied by alterations in lncRNA expression or function [8, 9], so lncRNAs have been shown to be able to serve as biomarkers for the diagnosis and/or therapy of gastric cancer [10]; the actual number of lncRNAs related to gastric cancer is unknown
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