Abstract
BackgroundIncreasing evidence has demonstrated that long non-coding RNAs (lncRNAs) play essential roles in the occurrence and development of human cancers, including gastric cancer (GC). However, the functional and clinical significance of lncRNAs are still poorly understood.MethodsIn this study, the expression of LncRNA HNF1A antisense RNA 1 (HNF1A-AS1) was first examined by lncRNAs microarray analysis in 6 GC tissues, and was then further verified by real-time quantitative reverse transcription PCR (qRT-PCR) both in 3 GC cell lines and 161 cases of GC tissues. We also evaluated the association between HNF1A-AS1 expression and clinicopathological features of patients with GC.ResultsLncRNAs microarray analysis results exhibited that HNF1A-AS1 was downregulated in GCs tissues (mean fold change 2.06, p < 0.05), which was further confirmed by qRT-PCR. The results from qRT-PCR showed that the expression of HNF1A-AS1 was not only downregulated in three GC cell lines (AGS, BGC-823, and MKN-45) relative to that in a normal gastric mucosal epithelial cell line (GES-1), but also decreased in GC tissues relative to that in paired adjacent non-neoplastic tissues (low expression, 94 of 161; low expression rate, 58.38 %). Furthermore, low HNF1A-AS1 expression was associated with tumor size/diameter (p = 0.005, multivariate analysis), levels of serum carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9), and RRM1 expression in tissue samples (p = 0.028, p = 0.009, and p = 0.006, respectively).ConclusionsTaken together, our data indicate that lncRNA HNF1A-AS1 may be a regulator of GC, and thus, it may have potential as a novel biomarker and treatment target for this type of cancer.
Highlights
Increasing evidence has demonstrated that long non-coding RNAs play essential roles in the occurrence and development of human cancers, including gastric cancer (GC)
IHC was performed by LncRNAs microarray analysis exhibits that hepatic nuclear factor 1 alpha gene (HNF1A)-AS1 expression is downregulated in GCs tissues As shown in Fig. 1, the results of long non-coding RNAs (lncRNAs) expression profile microarray analysis gotten from six GC tissues and their paired adjacent non-neoplastic gastric tissues exhibited that HNF1A-AS1 expression was downregulated in all of the six GC tissues, which indicated that this lncRNA may have the potential to be one of the tumor-related LncRNAs
QRT-PCR verification further confirms that HNF1A-AS1 expression is downregulated in GC cell lines and tissues We first investigated the expression of lncRNA HNF1AAS1 in three GC cell lines: AGS, BGC-823, and MKN-45
Summary
Increasing evidence has demonstrated that long non-coding RNAs (lncRNAs) play essential roles in the occurrence and development of human cancers, including gastric cancer (GC). Long non-coding RNAs (lncRNAs) are non-coding RNAs greater than 200 nucleotides [4] These RNAs do not encode proteins and are the main components of the human transcriptome [5]. Emerging evidence indicates that lncRNAs are important regulatory molecules whose main functions are related to modulation of gene expression networks [6]. Many of these networks are closely associated with cancer development and progression [6,7,8,9]. Data from different groups have determined that changes in expression of lncRNAs are related to the pathogenesis of diverse human cancers. Recent findings from Wang et al, Song et al, and Shao et al have demonstrated that changes in the expression profiles of lncRNAs are linked to gastric cancer carcinogenesis and progression and that lncRNAs could be used as early biomarkers or as treatment targets for GC [10–12]
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