Evaluation of the enzymatic activity of selected bacterial strains

Sławomir Wierzba,Adam Latała

doi:10.2478/v10026-007-0030-y

Abstract

Evaluation of the enzymatic activity of selected bacterial strains In these studies we attempted to evaluate the lipolytic, proteolytic and cellulolytic activity of bacterial strains isolated from water and the bottom sediments of Turawa Lake. The following bacterial genera prevailed among the isolated strains: Bacillus, Pseudomonas, Enterobacter, Cellulomonas and Cytophaga. The lipolytic activity was determined using a titrimetric method, the proteolytic activity — using a modified Anson method, and the cellulolytic activity - on the basis of mass decrement of a cellulose disk after 14 days of bacterial culture. The cultures were maintained at 28°C, pH 7.0 with the following substrates: olive oil, albumin and cellulose disk. Among the analysed microorganisms, Bacillus and Pseudomonas strains showed the highest lipolytic and proteolytic activity. In the cellulolytic assay Cytophaga bacteria showed about twofold higher activity than that of Celulomonas.

Highlights

Microorganisms play an important role in changes occurring in nature and are often used in technological processes
Microorganisms bring about the mineralisation of organic compounds of phosphorus and nitrogen, in consequence increasing the availability of biogens for phytoplankton
The purpose of the study conducted was to assess the lipolytic, proteolytic and cellulolytic activity of the selected bacterial strains isolated from the water and the bottom sediments of Turawa Lake

Summary

Evaluation of the enzymatic activity of selected bacterial strains

In these studies we attempted to evaluate the lipolytic, proteolytic and cellulolytic activity of bacterial strains isolated from water and the bottom sediments of Turawa Lake. The following bacterial genera prevailed among the isolated strains: Bacillus, Pseudomonas, Enterobacter, Cellulomonas and Cytophaga. The lipolytic activity was determined using a titrimetric method, the proteolytic activity — using a modified Anson method, and the cellulolytic activity – on the basis of mass decrement of a cellulose disk after 14 days of bacterial culture. The cultures were maintained at 28°C, pH 7.0 with the following substrates: olive oil, albumin and cellulose disk. Bacillus and Pseudomonas strains showed the highest lipolytic and proteolytic activity. In the cellulolytic assay Cytophaga bacteria showed about twofold higher activity than that of Celulomonas. Presented at VII Conference Wasteless Technologies and Waste Management in Chemical Industry and Agriculture, Miêdzyzdroje, 12 – 15 June, 2007

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION