Abstract

Two species of Chlorophyceae, Ankistrodesmus gracilis and Haemotococcus pluvialis, were used to compare and evaluate the effect of sugarcane molasses as a carbon source. Highest cell density in the two microalgae culture media was obtained in commercial culture media (CHU12 and WC). During exponential growth (day 1 to day 10), high cell density in H. pluvialis was detected for E. crassipes culture medium ranging between 0.4 x 105 cells mL-1 and 1.7 x 105 cells mL-1. Culture media were fundamental for growth under mixotrophic cultivation. Sugarcane molasses showed different results for the two microalgae with regard to growth performance, lipid and protein levels. Rates were high for H. pluvialis except lipid at the end of the experiment. In fact, A. gracilis presented almost double the lipid levels. Sugarcane molasses may be an alternative carbon source in laboratory conditions.

Highlights

  • The optimization and the reduction of production costs are crucial for the implementation of a successful algal crop

  • 10 days of cultivation, high cell density of Haematococcus pluvialis was observed in Eichhornia crassipes (EC) culture medium, ranging between 0.4 x 105 cells mL-1 and 1.7 x 105 cells mL-1, whilst in WC and NPK culture media ranged between 0.5 x 105 cells mL-1 and 1.3 x 105 cells mL-1 and between 0.3 x 105 cells mL-1 and 1 x 105 cells mL-1, respectively (Figure 1)

  • In EC alternative culture medium for H. pluvialis microalgae, the highest cell density was obtained during growth exponential phase

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Summary

Introduction

The optimization and the reduction of production costs are crucial for the implementation of a successful algal crop. Alternative sources, such as inorganic fertilizers, aquatic plants and sugarcane molasses, are being used to reduce production costs and increase algal biomass. In the case of the economic biomass cultivation of microalgae, it is necessary to explore the possibility of using a low-cost carbon source such as sugarcane molasses. Organic carbon sources such as glucose or acetate are usually expensive. Molasses consist of 50% total sugars, predominantly sucrose, since they contain significant amounts of reducing sugars such as glucose, fructose and other carbohydrates (Piasecka et al, 2017)

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