Evaluation of the effects of halloysite nanotube on polyhydroxybutyrate - chitosan electrospun scaffolds for cartilage tissue engineering applications

Abstract

Scaffolding method and material that mimic the extracellular matrix (ECM) of host tissue is an integral part of cartilage tissue engineering. This study aims to enhance the properties of electrospun scaffolds made of polyhydroxybutyrate (PHB) - Chitosan (Cs) by adding 1, 3, and 5 wt% halloysite nanotubes (HNT). The morphological, mechanical, and hydrophilicity evaluations expressed that the scaffold containing 3 wt% HNT exhibits the most appropriate features. The FTIR and Raman analysis confirmed hydrogen bond formation between the HNT and PHB-Cs blend. 3 wt% of HNT incorporation decreased the mean fibers' diameter from 965.189 to 745.16 nm and enhanced tensile strength by 169.4 %. By the addition of 3 wt% HNT, surface contact angle decreased from 61.45° ± 3.3 to 46.65 ± 1.8° and surface roughness increased from 684.69 to 747.62 nm. Our findings indicated that biodegradation had been slowed by incorporating HNT into the PHB-Cs matrix. Also, MTT test results demonstrated a significant increase in cell viability of chondrocytes on the PHB-Cs/3 wt% HNT (PC-3H) scaffold after 7 days of cell culture. Accordingly, the PC-3H scaffold can be considered a potential candidate for cartilage tissue engineering.