Abstract
Various pathological conditions can be characterized not only by a decrease or increase in basal levels of hydrogen sulfide in the serum, but also the levels of hydrogen sulfide can modulate the course of the pathological process. The impact of serum hydrogen sulfide on the condition of the intact vaginal wall of rats was evaluated in this study. The aim of the study was to evaluate the effect of excess and deficiency of serum hydrogen sulfide on the condition of the vaginal wall of intact rats. The study was performed on 75 female Wistar rats under 1 year of age and weighing 160.0 to 200.0 grams. All animals were divided into 6 groups: control (intact rats); experimental 1 (H2S excess); experimental 2 (H2S deficiency); experimental 3 (intravaginal administration of suppositories with clindamycin); experimental 4 (H2S excess + suppositories with clindamycin); experimental 5 (H2S deficiency + suppositories with clindamycin). The levels of serum hydrogen sulfide were studied, as well as microscopic examination of the structure of the vaginal wall and determination of the levels of TNF-α and IL-1β in tissue homogenate were performed. In experimental groups 3, 4 and 5 all studies were performed in dynamics – 10 minutes, 4, 8 and 24 hours after a single intravaginal administration of clindamycin phosphate. The data were processed using the statistical software package SPSS 20.0 for Windows. Under conditions of both hydrogen sulfide deficiency and excess, no statistically significant changes in TNF-α and IL-1β levels in the vaginal wall of intact rats were observed. Also, no changes in the histological structure of the wall were found. Similar data were demonstrated in experimental groups 3, 4 and 5. This picture is explained by the fact that hydrogen sulfide affects various parts of the inflammatory process, while reducing the production of inflammatory mediators. In intact tissues, in the absence of an inflammatory process, there is no point of application of hydrogen sulfide, and therefore no significant changes are observed. Thus, both excess and deficiency of serum hydrogen sulfide do not affect the condition of the vaginal wall of intact rats.
Highlights
Despite the constant progress in clinical microbiology and pharmacology of antibacterial drugs, inflammatory diseases of the lower genital tract continue to occupy a leading place in the structure of obstetric and gynecological pathology
All rats were randomly divided into 6 groups: control group (n=5) - intact animals; experimental group 1 (n=5) - rats with excess hydrogen sulfide; experimental group 2 (n=5) - rats with hydrogen sulfide deficiency; experimental group 3 (n=20) - rats, which were intravaginally administered clindamycin phosphate in the form of suppositories; experimental group 4 (n=20) - rats with excess hydrogen sulfide, which were intravaginally administered clindamycin phosphate in the form of suppositories; experimental group 5 (n=20) - rats with hydrogen sulfide deficiency, which were intravaginally administered clindamycin phosphate in the form of suppositories
Groups with intravaginal administration of clindamycin phosphate in the form of suppositories were included in the study, as this antibiotic is the drug of choice and an integral part of the comprehensive treatment of inflammatory diseases of the vagina [14, 27]
Summary
Despite the constant progress in clinical microbiology and pharmacology of antibacterial drugs, inflammatory diseases of the lower genital tract continue to occupy a leading place in the structure of obstetric and gynecological pathology. The most significant of these is bacterial vaginosis [1, 15] This pathology is a serious health problem for women of reproductive age, their children and partners, as bacterial vaginosis is associated with adverse effects on reproductive health, such as pelvic inflammatory disease, miscarriage, premature birth, and can lead to increased risk of human immunodeficiency virus [7, 22, 23]. The social and practical significance of this pathology encourages the search for new mechanisms of control and modulation of the inflammatory process in the lower genital tract. One such modulator of the inflammatory process is hydrogen sulfide. Hydrogen sulfide, which from the beginning was considered only as a highly toxic exogenous product of protein breakdown [13], is recognized as one of the important endogenous factors in maintaining homeostasis
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