EVALUATION OF THE CONTENT OF VITAMINS IN MICROGREENS OF SEVERAL SPECIES OF CULTIVATED PLANTS

Abstract

In the course of the study, the assessment of the content of fat-and water-soluble vitamins in microgreens of five species of cultivated plants at different stages of its development was carried out. For analysis, microgreens were grown in plastic containers on a nonwoven viscose support. After the seed was laid, they were placed in a climatic chamber with a program that simulates the natural conditions of daily cycles. Collection of samples was started after massive cotyledonous leaf opening and repeated every other day until commercial maturity of the product was reached. Then they were frozen at a temperature of about -18 °C and kept in this state until the study. Before analysis, the plant material was thawed and, without drying, was ground to fragments with sizes of 1–3 mm. Aqueous and isopropanol extracts obtained from precise weighed portions of the studied plant material were analyzed. Determination of the content of fat- and water-soluble vitamins was carried out by the method of liquid tandem chromatomass spectrometry on a device with a system of three quadrupoles. In the course of the study, a fairly high content of some vitamins was found in the composition of microgreen samples. With the course of its development, the concentration of the determined components also changes: both accumulation and their consumption are observed, and in some cases – fluctuation. Further research will allow you to select the most optimal parameters for growing microgreens and develop recommendations for the timing of its use when the concentration of vitamins is maximum.