Abstract
Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined.
Highlights
According to data reported by Turai [1], 134 deaths were registered after 420 radiation accidents worldwide between 1944 and 2002
No significant difference was found between the male and female donors, and the same results were found between the two experiments; we pooled all of the data into one database to describe the dose-response relationship
To assess the potential use of the comet assay in radiation biodosimetry, the DNA repair kinetics should be considered in the dose-response curve
Summary
According to data reported by Turai [1], 134 deaths were registered after 420 radiation accidents worldwide between 1944 and 2002. The chromosome aberration assay introduced by Bender and Gooch [5] remains the “gold standard” for early-response accident biodosimetry and definitive dose assessment [6], but it is time-consuming for the purpose of rapid response because the lymphocyte culturing process takes approximately 48 h to 72 h. Rapid and sensitive methods are needed to assess the DNA damage induced by ionizing radiation. The comet assay is a rapid and sensitive microdosimetric technique that may be suitable for in vivo human biomonitoring, especially in cases of incidental exposure to ionizing radiation [14,15,16]. To fit the dose- and time-response curves after in vitro radiation, the difference in the number of DNA double-strand breaks (DSBs) in lymphocytes between in vivo and in vitro radiation exposures should be studied first
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