Evaluation of the association between P53 codon 72 and P21 codon 31genetic polymorphisms within gastric and colorectal cancer risk in a Moroccan Cohort

Abstract

AimThe aim of the current study was to explore the association between the p53 (Arg > Pro) (rs1042522) and p21 (Ser > Arg) (rs1801270) polymorphisms and gastric and colorectal cancers susceptibility among Moroccan population. MethodsThis case control study was conducted on 70 gastric cancer, 70 colorectal cancer patients and 70 healthy patients. The p53 (Arg > Pro) and p21 (Ser > Arg) polymorphisms were analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) assay. ResultsFor the P53 codon 72 the genotype frequencies were 27.1% for Arg/Arg, 65,7% for Arg/Pro and 7,1% for Pro/Pro in control group, and 40%; 17,1% for Arg/Arg, 34.3%; 42,9% for Arg/Pro and 25,7%; 40% for Pro/Pro respectively in the gastric and colorectal cancer patients. Genotype frequencies of the p21 codon 31 were 17.1% for Arg/Arg, 71.4% for Ser/Arg and 11.4% for Ser/Ser in control group, and 22.9%; 25.7% for Arg/Arg, 35.7%; 41.4% and 41.4%; 32.9% for Ser/Ser respectively in the patient with gastric and colorectal cancer. This difference was statistically significant for the Pro/Pro genotype. The same trend as observed in the Pro allele distribution for colorectal cancer patients (P = 0.0004). No statistically significant correlation was found between p21 (Ser > Arg) and the development of gastric and colorectal cancers. ConclusionOur results have demonstrated that the P53 (rs1042522) polymorphism may contribute to the risk of colorectal cancer development while the P21 (rs1801270) polymorphism does not contribute to this susceptibility for both cancers in the Moroccan population.