Evaluation of the Adhesion of Streptococcus Mutans to Different Orthodontic Arch Wires and the Effect of Honey Mouth Wash on it

Abstract

Purpose: To compare the adhesion of Streptococcus Mutans to different orthodontic arch wires and also to determine the role of honey as mouth wash during orthodontic treatment. Materials and methods: 21 female orthodontic patients their age ranges from18-23 were participated in this study. Nitinol and Stainless steel half arch wires were inserted separately on each half of maxillary arch (split mouth design) for 90 minute before using honey mouth wash, then for another 90 minute 3, 7, 14 and 30 days after using honey mouth wash 2 times per day. On each time, samples of wires which about 10 mm from the distal part of each wire were been cut. The wire samples were placed on Eppendorf tubes and cultured on Mitis Salivarius media in the candle jar 5-10% CO2 at 37˚C for 2-3 days. Then the amount of Streptococcus Mutans adhesion were measured by Counting of colony forming units Results: Streptococcus Mutans adhesion were higher on Stainless steel wires than Nitinol wires and also were lower at both wires after using honey mouthwash. Statistical analysis revealed that there was a statistically significant difference between Stainless steel and Nitinol wires and after use of honey mouthwash (P <0.05). Conclusion: Streptococcus Mutans adhesion was higher on Stainless steel wires than Nitinol wires. Honey can be used as a natural antibacterial mouthwash during orthodontic treatment and also should be supported by other studies.