Evaluation of Techniques That Measure Hydrogen and Methane Levels in Breaths of Horses with Gastrointestinal Diseases

Abstract

Techniques for measuring hydrogen (H2) and methane (CH4) levels in breaths were assessed in horses with gastrointestinal (GI) diseases. A total of 31 horses were studied: 11 healthy horses (Group A), 10 horses with GI diseases (Group B), and 10 horses with diseases without GI involvement (Group C). Expired gases were measured using gas chromatography. CH4 levels in Group A were significantly lower than those in Group B. In Group B, CH4 levels were significantly higher after treatment. Fluctuations in H2 and CH4 levels in breath were associated with changes in GI function.