Evaluation of Targeted Liposomes

Abstract

Filtration is the foremost reasonable sterilization method for thermo-labile liposomes because it does not incorporate any heat or any extreme condition that can result in the corruption of the structure of the liposomes or leakage of the encapsulated molecule. In addition to these, filtration has a few drawbacks such as necessity of anaseptic area, manufacturing equipment resistant to higher pressure values, and being only appropriate to the liposomes that are smaller than 200 nm in diameter. Methods used to assess the amount of material encapsulated inside liposomes usually depend on the lipid bilayer destruction and subsequent quantification of the released content. When evaluating the morphology of liposomes, the characterization of the structure, shape, surface morphology, and size of liposomes must be considered essentially. Evaluation of the stability of liposomal preparations, the in vivo kinetics of these systems, and their ability to target desired cells/tissue significantly depend on the size distribution of liposomes.