Evaluation of Synergistic In vitro Anti-inflammatory Activity of Eulophia ochreata Lindl and Zingiber cassumunar Roxb

Abstract

Medicines derived from plant extracts are being increasingly utilized to treat a variety of diseases, though relatively little knowledge about their activity is available. Based on survey, herbs used for the study has well established histories of human use for the treatment of arthritic conditions. The present research paper highlights the invitro anti-inflammatory activity of Eulophia ochreata Lindl, Zingiber cassumunar Roxb and its 1:1 blend of both the extracts. The test extracts of varying concentrations were incubated with egg albumin under controlled experimental conditions and subjected to determination of absorbance to assess the anti-inflammatory property. The results obtained exhibited a concentration- dependent inhibition of protein denaturation by both extracts, including the 1:1 blend of the extracts. Membrane stabilization activity was evaluated using the human red blood cells(HRBC) membrane stabilization method as HRBC membranes are similar to lysosomal membrane components. The prevention of HRBC membrane lysis was taken as a measure of anti-inflammatory activity of test extracts including the 1:1 blend of the extracts. A standard anti-inflammatory drug, diclofenac, was used as reference drug. From the present findings it can be concluded that both Eulophia ochreata Lindl and Zingiber cassumunar Roxb and its 1: 1 bend of extract possessed marked anti-inflammatory effect .Thus 1:1 bend of extract being more effective. Hence, there is urgent need to utilize ancient knowledge of herbal plants and its synergistic activity to bring its maximum potential in the field of medical and pharmaceutical sciences in novel herbal drug development which will economically benefited for common man.