Abstract
 Trizol extraction for polysaccharide-rich plant tissue was unsuitable for isolating total RNA from loquat fruit. CTAB-LiCl extraction was improved with pretreatment using washing buffer with 80% ethanol and 70% acetone. The RNA isolated by this protocol from different loquat fruit tissue at various development periods, was high in purity (A260/A280 ratio ranged from 1.81 to 1.99 and A260/A230 ratio was over 2.0), and high in yield.
Highlights
Loquat (Eriobotrya japonica Lindl, Rosaceae, subfamily Maloideae), is indigenous to China, but is cultivated worldwide in suitable climates
Peel and pulp of young and ripe fruit as materials were evaluated with six methods of total RNA extraction
With method 1, the total RNA of the young fruits had A260/A230 ratios lower than 2.0, suggesting that RNA samples were little contaminated by polysaccharides, proteins, DNA, phenol or salts
Summary
Loquat (Eriobotrya japonica Lindl, Rosaceae, subfamily Maloideae), is indigenous to China, but is cultivated worldwide in suitable climates. Loquat contains high level of polysaccharides, proteins, and secondary metabolites such as polyphenols (Liu et al, 2005) that cause degradation and low yield of functional mRNA (Wang et al, 2000). Six methods for total RNA extraction of loquat fruit were compared and evaluated
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call