Abstract
Six assays were evaluated in this study to determine their suitability for the diagnosis of acute dengue infection using samples from 259 Sri Lankan patients with acute fevers (99 confirmed dengue cases and 160 patients with other confirmed acute febrile illnesses): (i) the Merlin dengue fever IgG & IgM combo device (Merlin), (ii) the Standard Diagnostics Dengue Duo nonstructural 1 (NS1) antigen and IgG/IgM combo device (Standard Diagnostics, South Korea), (iii) the Biosynex Immunoquick dengue fever IgG and IgM (Biosynex, France) assay, (iv) the Bio-Rad NS1 antigen strip (Bio-Rad, France), (v) the Panbio Dengue Duo IgG/IgM Cassette (Inverness, Australia), and (vi) the Panbio dengue NS1 antigen strip (Inverness, Australia). The median number of days of fever prior to admission sample collection was 5 days (interquartile range, 3 to 7 days). Sensitivity and specificity of the NS1 antigen tests ranged from 49 to 59% and from 93 to 99%, respectively, and sensitivity and sensitivity of the IgM antibody test ranged from 71 to 80% and from 46 to 90%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics Dengue Duo test gave the best compromise of sensitivity and specificity (93% and 89%, respectively) and provided the best sensitivity in patients presenting at different times after fever onset. The Merlin IgM/IgG antibody tests correctly classified 64% and 86% of the primary and secondary dengue infection cases, respectively, and the Standard Diagnostics IgM/IgG antibody tests correctly classified 71% and 83% of the primary and secondary dengue infection cases, respectively. This study provides strong evidence of the value of combining dengue antigen- and antibody-based test results in the rapid diagnostic test (RDT) format for the acute diagnosis of dengue.
Highlights
Both reference serology and RT-PCR assays were performed by Armed Forces Research Institute of Medical Sciences (AFRIMS) staff who were blind to the results of the dengue virus nonstructural 1 (NS1) antigen or IgM capture enzyme-linked immunosorbent assay (ELISA) conducted at MORU
This study evaluated six commercially available rapid diagnostic test (RDT) that use IgM antibodies and NS1 antigens, individually or in combination, for the diagnosis of acute dengue infections in the tropics
The recent introduction of dengue virus NS1 antigen detection assays has brought a great deal of promise to the area of acute dengue diagnosis
Summary
Dengue virus infections were confirmed on an individual patient basis, with the paired admission and convalescent-phase specimens tested by the AFRIMS with IgM and IgG antibody capture ELISAs [12], using the following interpretations. An RT-PCRpositive result was used to determine serotype identity, but these results were not used as part of the AFRIMS diagnostic algorithm Both reference serology and RT-PCR assays were performed by AFRIMS staff who were blind to the results of the dengue virus NS1 antigen or IgM capture ELISAs conducted at MORU. Diagnostic accuracy was calculated for the dengue virus rapid tests by comparing the interpretation of the most experienced individual reader (K.J., with 5 years of experience) to the final patient diagnosis (i.e., dengue positive or dengue negative) based on the results of reference serology.
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