Diagnostic biologique direct précoce de la dengue par détection génomique du virus avec RT-PCR (transcription inverse et amplification génique par réaction de polymérisation en chaîne)

Abstract

To conduct a retrospective analysis of dengue cases in Kolkata, on the basis of presence of anti-dengue IgM in their sera and presence or absence of anti-dengue IgG and dengue specific Non structural 1 (NS1) antigen in each of the serum sample.Sample was tested quantitatively employing ELISA technique, using Biorad test kits, with a view to get a more comprehensive picture of dengue in an urban endemic area and also to evaluate individual cases.This reconstructed study revealed that of those 91 dengue cases, 70.3% (64) and 29.7% (27) were suffering from secondary and primary dengue respectively, showing that number of secondary dengue cases were much more than that of primary dengue cases with a possibility of emergence of DHF. A small proportion of cases 18.7% (17) were reactive for NS1. The duration of fever in NS1 antigen positive cases varied between 5 and 7 days. Of 17 NS1 reactive cases, 10 (10.9%) and 7 (7.7%) were suffering from secondary and primary dengue respectively.Early detection of primary and secondary dengue cases would be facilitated by utilizing all three parameters (NS1 antigen, anti-dengue IgM and IgG) helping to evaluate, monitor and treat a dengue case effectively.