Abstract
ABSTRACTOsteogenic potential of autologous bone marrow aspirates was evaluated on transverse tibia fractures of 12 Nigeria indigenous dogs presented to University of Nigeria Veterinary Teaching Hospital, Nsukka (UNVTH). They were assigned into four groups: single application group (Group1; n = 3), double application group (Group 2; n = 3), triple application group (Group 2; n = 3) and the control group (Group 3; n = 3). The fractures were immobilized with Steinmann intramedullary pins in a normograde manner. The patients were then treated as follows: Group 1: with autologous bone marrow aspirate on the day of surgery; Group 2: with autologous bone marrow aspirate on days 0 and 14 post surgery; Group 3: with autologous bone marrow aspirate on days 0, 14 and 28 post surgery; Group 4: 1 ml normal saline was applied on the fracture site. Post-treatment radiographs of the fractures were taken on weeks 1, 2, 4, 7 and 10. The rate of healing was compared using radiographic union scale for tibia scores. The results showed a significant difference (p < .05) in the healing of the treated groups and the control at week 7 post surgery. There was however no significant (p > .05) difference in the healing of the fractures with single and the multiple applications.
Highlights
Successful bone formation is the key to fracture healing
Previous studies on fracture healing and the repair of segmental bone defects focused on bone matrix substitutes (Grundel et al 1991; Delloye et al 1992; Gogolewski et al 2000)
Kitoh et al (2004) reported an encouraging result of local injection of both culture expanded bone marrow aspirates and platelet-rich plasma to achondroplasia patients
Summary
Previous studies on fracture healing and the repair of segmental bone defects focused on bone matrix substitutes (Grundel et al 1991; Delloye et al 1992; Gogolewski et al 2000). These substitute matrices did not perform as well as autograft for several reasons, including histochemical responses by the host tissue and a dearth of living cells. Kitoh et al (2004) reported an encouraging result of local injection of both culture expanded bone marrow aspirates and platelet-rich plasma to achondroplasia patients. Culture expansion technique requires additional visit to the clinic, special equipment and additional training to harvest the stem cells, which may not be feasible in most under developed countries of the world, including Nigeria
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