Abstract
ABSTRACTSeveral methods of preparing homogenates from potato tuber for estimating the total activity of polyphenol oxidase (PPO) were compared. To remove endogenous phenols that may inhibit PPO, homogenates were either treated with polyvinylpyrrolidone (PVPP) or subjected to extensive dialysis, ammonium sulfate or acetone precipitation or Sephadex G‐25 chromatography. The G‐25 gel filtration method was superior in removing phenolic compounds and in retaining PPO activity. Using this chromatographic method, the tubers of Russet‐Burbank cultivar were found to contain highest PPO activity followed by Russett and then Atlantic cultivars.
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