Abstract
Serum electrophoresis in cellulose acetate strip is a technique commonly used to separate the protein fractions. This technique allows detecting quantitative and qualitative changes in the serum proteins associated with different diseases. The aim of this study was to characterize the changes of the serum protein fractions produced in sheep suffering from babesiosis. Blood samples were collected from 52 healthy and naturally infected sheep with Babesia ovis. Serum total proteins were calculated, and serum electrophoresis was performed. The parasitological diagnosis was confirmed using polymerase chain reaction assay by amplifying a partial small subunit ribosomal RNA gene sequence of B. ovis. Mean values from total proteins and globulin fractions were significantly higher (P < 0.05) than that observed for the healthy group. Compared to the healthy group, significant decrease (P < 0.05) in the mean percentage of albumin in the infected group was determined. Results obtained in this study showed that B. ovis infection has caused albumin reduction and increased serum total protein and globulin level.
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