Evaluation of serum levels of interferon beta (INF-B) and Nucleotide-binding oligomerization domain 2 (NOD2) gene polymorphism in children with atopic asthma phenotype

Abstract

Background People of all ages are primarily affected by the chronic inflammatory disease asthma, which is most common in children. A modified International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire found 7.7% of Nile Delta schoolchildren had asthma. This research was to show is their association between the underlying gene polymorphisms of NOD2 and serum level of INF beta and atopic asthma phenotype in Egyptian children and evaluate is their relationship between NOD2 gene polymorphism and the serum level of INF beta. Methods The 127 asthmatic children in this case-control study had typical asthma symptoms, and the 39 age- and sex-matched healthy controls. Subjects were divided in to three groups: Atopic asthma phenotype group: included 60 asthmatic children who had allergic rhinitis and atopic dermatitis as a predominant symptom, Wheezy phenotype group: included 67 asthmatic children presented predominantly with wheezes and controls: 39 healthy controls were chosen because they appeared to be in good health, had no history of symptoms similar to those of asthma, and had no relatives who had allergies or asthma. Results Total serum IgE and eosinophil percentage in control groups showed a positive correlation (r=0.640, P=0.006). In asthmatics, serum INF-β and IgE correlated positively (r=0.217, P=0.031). Increased Serum eosinophilic percentage was associated with decreased serum INF-β and increased serum IgE. Conclusions In comparison to the wheezy phenotype group, the percentage of eosinophils is significantly higher in the atopic asthma phenotype. Serum INF-β and IgE levels went up in tandem with the percentage of eosinophils in the blood. rs2066845 NOD2 genotype The GG genotype increased the risk of asthma in general but not the type of asthma.