Abstract
A new commercial anti-Japanese encephalitis virus IgM and IgG indirect immunofluorescence test (IIFT) was evaluated for the detection of the humoral immune response after Japanese encephalitis vaccination. The IgM IIFT was compared to two IgM capture ELISAs and the IgG IIFT was analysed in comparison to a plaque reduction neutralization test (PRNT50) and an IgG ELISA. Moreover, the course of the immune reaction after vaccination with an inactivated JEV vaccine was examined. For the present study 300 serum samples from different blood withdrawals from 100 persons vaccinated against Japanese encephalitis were used. For the IgM evaluation, altogether 78 PRNT50 positive samples taken 7 to 56 days after vaccination and 78 PRNT50 negative sera were analyzed with the Euroimmun anti-JEV IgM IIFT, the Panbio Japanese Encephalitis – Dengue IgM Combo ELISA and the InBios JE Detect IgM capture ELISA. For the IgG evaluation, 100 sera taken 56 days after vaccination and 100 corresponding sera taken before vaccination were tested in the PRNT50, the Euroimmun anti-JEV IgG IIFT, and the InBios JE Detect IgG ELISA. The Euroimmun IgM IIFT showed in comparison to the Panbio ELISA a specificity of 95% and a sensitivity of 86%. With respect to the InBios ELISA, the values were 100% and 83.9%, respectively. The analysis of the Euroimmun IgG IIFT performance and the PRNT50 results demonstrated a specificity of 100% and a sensitivity of 93.8%, whereas it was not possible to detect more than 6.6% of the PRNT50 positive sera as positive with the InBios JE Detect IgG ELISA. Thus, the IIFT is a valuable alternative to the established methods in detecting anti-JEV antibodies after vaccination in travellers and it might prove useful for the diagnosis of acutely infected persons.
Highlights
Japanese encephalitis virus (JEV), a mosquito-borne pathogen of the genus Flavivirus, family Flaviviridae, is the main cause of viral encephalitis in Asia
300 different sera were chosen for the present study as described below: The samples from V5 (n = 100) were analyzed for IgG with the Euroimmun anti-JEV IgG indirect immunofluorescence test (IIFT) and with the InBios JE Detect IgG ELISA
For evaluation of the anti-JEV IIFT we examined IgM and IgG antibodies in the serum panels using the Titerplane technique as described in the instruction manual
Summary
Japanese encephalitis virus (JEV), a mosquito-borne pathogen of the genus Flavivirus, family Flaviviridae, is the main cause of viral encephalitis in Asia. Three billion people live in the endemic areas and at least 50,000 clinical Japanese encephalitis (JE) cases occur each year, which are a great burden to the affected populations [1,2]. JEV is widespread throughout Asia up to the northern tip of Australia [3,4] and is considered as an emerging or re-emerging virus [5]. It is transmitted by bloodsucking Culex mosquitoes predominantly Culex tritaeniorhynchus. Most cases occur in rural areas, but transmission is found in peri-urban and urban centres [6]. The disease can only be treated symptomatically but different vaccines are available for preventing it
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
