Evaluation of semen quality brought from Kundasale as replacement of locally produced semen for artificial insemination in Jaffna Peninsula, Sri Lanka

K Nilani,T Eswaramohan,P Mahadhevan

doi:10.4038/vingnanam.v11i1.4112

K Nilani, T Eswaramohan + Show 1 more

Open Access

https://doi.org/10.4038/vingnanam.v11i1.4112

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Journal: Vingnanam Journal of Science	Publication Date: Jun 7, 2015
License type: cc-by

Affiliation: University of Jaffna

Abstract

Artificial Insemination (AI) with frozen semen has been proved as the most potent method for rapid genetic improvement in domestic animals. While the use of AI with fresh semen is limited by the restricted period of sperm viability, this limitation may be overcome by the storage of frozen semen. Recently AI center of Thirunelvely is being advised to use the deep-frozen semen processed from Kundasale of Central Province (CP) for AI purposes. Thus the present study was undertaken to compare the quality of locally produced stored semen (LPS) and semen brought from Kundasale in deep-frozen condition (KDFS) by assessing the viability of them as the prominent factor that determine the successful of AI service . A batch of LPS and KDFS was obtained from the AI center at Thirunelvely. LPS was diluted with 2.9% sodium citrate buffer solution to evaluate the individual progressive motility and sperm velocity, sperm morphology, and acrosome integrity. Sperm smear was prepared by using 1% eosin and 0.75% Giemsa stain to identify intact and reacted acrosome and sperm abnormalities respectively. Thawed semen was also assessed for motility examination, percentage of intact acrosome, viability and morphology. Paired‘t’ test was performed to compare the means of the viability of LPS and KDFS (P Ving. Journal of Science 2012, Vol. 11, No. 1, 19-25

Highlights

Artificial Insemination (AI) with frozen semen has been proved as the most potent method for rapid genetic improvement in domestic animals
While the use of AI with fresh semen is limited by the restricted period of sperm viability, this limitation may be overcome by the storage of frozen semen
AI with locally produced stored semen (LPS) has been performed at AI center, Thirunelvely in Jaffna peninsula since 1957, recently this AI center is being advised to use the deep-frozen semen processed from Kundasale (KDFS) of Central Province (CP) for AI purposes

Summary

Introduction

Artificial Insemination (AI) with frozen semen has been proved as the most potent method for rapid genetic improvement in domestic animals. While the use of AI with fresh semen is limited by the restricted period of sperm viability, this limitation may be overcome by the storage of frozen semen. Thereby the main goal of semen evaluation is to predict its fertilizing ability [4] and several cell staining techniques have been developed to evaluate sperm damages caused by cryopreservation, such as the eosinnigrosin staining. The surviving sperm population might have morphological defects that reduce and impairs their fertilizing ability, and it is well known that cryopreserved semen presents sub lethal damages and fertility reduction [5]. AI with locally produced stored semen (LPS) has been performed at AI center, Thirunelvely in Jaffna peninsula since 1957, recently this AI center is being advised to use the deep-frozen semen processed from Kundasale (KDFS) of CP for AI purposes. The present study was undertaken to compare the quality of LPS and KDFS by assessing the viability of them as the prominent factor that determine the successful of AI service

MATERIAL AND METHODS

RESULTS AND DISCUSSION

CONCLUSION