Evaluation of Saccharum officinarum L. bagasse-derived polysaccharides as native immunomodulatory and anticoccidial agents in broilers

Abstract

Coccidiosis is one of the most important protozoal diseases of the poultry industry, inflicting heavy economic losses in the form of high mortality and morbidity in affected birds. Under these circumstances, the development of nonchemical consumer-friendly strategies for its effective control is of paramount importance. Therefore, this study was conducted to evaluate the sugarcane (Saccharum officinarum L.) bagasse-derived polysaccharides as native immunomodulatory and anticoccidial agents in commercial broilers. Polysaccharides were recovered from sugarcane bagasse (PSCB) and analyzed by high-performance liquid chromatography (HPLC). Five different sugars including melezitose, maltose, glucose, mannose, and fructose were detected in hydrolyzed solution of PSCB. The isolated PSCB were orally administered to the broilers in three graded doses ranging from 10 to 50mg/kg of body weight/day for 3 consecutive days, i.e., fifth through seventh days of life. Results showed significantly enhanced (p<0.05) lymphoproliferative and humoral responses to T-cell mitogen (PHA-P) and sheep red blood cells (SRBCs) in PSCB-administered chickens. In a challenge experiment, percent protection and daily weight gains were significantly higher (p<0.05), whereas mean oocyst counts and lesion scores were lower (p<0.05) in PSCB-administered chickens as compared to control. ELISA showed that PSCB significantly enhanced (p<0.05) antibody titers against the Eimeria species used for the induction of infection in chickens of PSCB-administered and control groups. In conclusion, PSCB showed the potential to modulate the immune responses in industrial broiler chickens with subsequent protection against coccidial infection.