Abstract

SUMMARY A field strain (87-8363) of bovid herpesvirus-4 (bhv-4) isolated from an aborted bovine fetus was used to inoculate pregnant rabbits. Eleven rabbits in midgestation were alloted to 4 groups consisting of 3 infected groups and 1 control group. Rabbits were inoculated with bhv-4 or mock-infected cell culture preparations via iv, intravaginal, and intrauterine routes. Mild vulvovaginitis and endometritis were observed after intravaginal and iv inoculation of bhv-4, whereas intrauterine inoculation of bhv-4 resulted in abortion of hemorrhagic fetuses and nonsuppurative endometritis. Virus was successfully isolated from organ explants of fetal tissues. Rabbits seroconverted 1 week after infection as detected by results of an indirect immunofluorescence assay.

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