Evaluation of phenolic and anthraquinone profiles of Polygonacaeae family from Turkey

Abstract

The purpose of this research is to compare the content of phenolic compounds (catechin, esculetin, polydatin, rutin, resveratrol, quercetin) and anthraquinones (aloe-emodin, emodin, chrysophanol, physcion) in Polygonum cognatum Meissn. and Rumex crispus L. plant taxa from the flora of Turkey. Extraction parameters such as solvent, solvent ratio, and time for soxhlet method were optimized, and the most effective extraction was carried out for eight hours with methanol-water (3:1 v/v). The development and validation of an HPLC-DAD method allowed for the simultaneous quantification of phenolic compounds and anthraquinones in the plant extracts. The analytical method conditions were defined through an investigation of various parameters, including mobile phase composition, flow rate, and column temperature. The method was validated with good linearity, precision, sensitivity, and recovery. The LOD and LOQ values of the compounds were in the range of 0.032–0.457 µg/mL and 0.106–1.523 µg/mL, respectively. The findings of the study showed that this analytical method was successfully applied to identify and measure the phenolic and anthraquinone compounds in Polygonum cognatum Meissn. and Rumex crispus L. plants.